Optical methods for illumination of dyed biological samples that compute the RGB complementary color in an RGB-HSI-RGB color space transformation.

Patent No. NC2018/0007279, Colombia (Granted).
health, biology, medicine (pathology, ophthalmology, microscopic endodontics), medical diagnostics (colposcopy, endoscopy, otoscopy), laboratories.
Intelligent illumination that automatically selects the color that maximizes the contrast of the sample; easy attachment to the viewing instrument; uses a polychromatic LED module; viewing of biological samples by electronic generation of complementary color, user interface that allows selection of the mode of use: assisted or automatic; control of color, intensity and contrast; can be controlled by a mobile device or remotely via the Internet.
  • Improves the visualization of biological samples stained with histological dyes such as.
  • Hematoxylin – Eosin (H – E), among others.
  • It allows the visualization and contrast of the tissue area of interest through endoscopy, and in similar applications where the visualization of the sample is enhanced by illuminating with light of a specific color chosen by the examiner or by the system.
  • Can generate any color in the visible range.
  • Maximizes contrast by automatically calculating the complementary color.
  • It has two modes of use: manual or automatic.
  • Reduces and optimizes observation and diagnosis times.
  • It can be easily attached to the viewing instrument.
Universidad del Cauca.